A single-volume manga sequel, Blood: The Last Vampire 2000 written by Benkyo Tamaoki, was published in Japan in 2001 by Kadokawa Shoten, and in English by Viz Media in November 2002 with the title slightly modified to Blood: The Last Vampire 2002. Three Japanese light novel adaptations have also been released for the series, along with a video game. It also spawned a fifty-episode anime series, Blood+, which is an alternate universe story.

The story is set in the American Yokota Air Base located in post-WWII Japan, a few months before the beginning of the Vietnam War. Its main protagonist is a girl named Saya, who hunts hematophagous bat-like creatures called chiropterans for a secret organization known as the Red Shield.